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Human IL-3 stimulates endothelial cell motility and promotes in vivo new vessel formation.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1999 Aug 15; Vol. 163 (4), pp. 2151-9. - Publication Year :
- 1999
-
Abstract
- Angiogenesis is a critical process for growth of new capillary blood vessels from preexisting capillaries and postcapillary venules, both in physiological and pathological conditions. Endothelial cell proliferation is a major component of angiogenesis and it is regulated by several growth factors. It has been previously shown that the human hemopoietic growth factor IL-3 (hIL-3), predominantly produced by activated T lymphocytes, stimulates both endothelial cell proliferation and functional activation. In the present study, we report that hIL-3 is able to induce directional migration and tube formation of HUVEC. The in vivo neoangiogenetic effect of hIL-3 was also demonstrated in a murine model in which Matrigel was used for the delivery of the cytokine, suggesting a role of hIL-3 in sustaining neoangiogenesis. Challenge of HUVEC with hIL-3 lead to the synthesis of platelet-activating factor (PAF), which was found to act as secondary mediator for hIL-3-mediated endothelial cell motility but not for endothelial cell proliferation. Consistent with the role of STAT5 proteins in regulating IL-3-mediated mitogenic signals, we herein report that, in hIL-3-stimulated HUVEC, the recruitment of STAT5A and STAT5B, by the beta common (betac) subunit of the IL-3R, was not affected by PAF receptor blockade.
- Subjects :
- Animals
Apolipoproteins D
Azepines pharmacology
Carrier Proteins metabolism
Cell Division drug effects
Cell Line
Cell Movement drug effects
DNA-Binding Proteins antagonists & inhibitors
DNA-Binding Proteins metabolism
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Endothelium, Vascular metabolism
Female
Humans
Injections, Subcutaneous
Interleukin-3 metabolism
Macromolecular Substances
Mice
Mice, Inbred C57BL
Neovascularization, Physiologic drug effects
Platelet Activating Factor antagonists & inhibitors
Platelet Activating Factor biosynthesis
Platelet Membrane Glycoproteins antagonists & inhibitors
Protein Binding
Receptors, Interleukin-3 metabolism
STAT5 Transcription Factor
Trans-Activators antagonists & inhibitors
Trans-Activators metabolism
Triazoles pharmacology
Tumor Suppressor Proteins
Apolipoproteins
Cell Movement physiology
Endothelium, Vascular physiology
Glycoproteins
Interleukin-3 administration & dosage
Interleukin-3 physiology
Membrane Transport Proteins
Milk Proteins
Neovascularization, Physiologic physiology
Receptors, Cell Surface
Receptors, G-Protein-Coupled
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 163
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 10438956