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Modification of the mitochondrial sulfonylurea receptor by thiol reagents.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1999 Aug 19; Vol. 262 (1), pp. 255-8. - Publication Year :
- 1999
-
Abstract
- The purpose of this study was to investigate the effects exerted by thiol-modifying reagents on themitochondrial sulfonylurea receptor. The thiol-oxidizing agents (timerosal and 5, 5'-dithio-bis(2-nitrobenzoic acid)) were found to produce a large inhibition (70% to 80%) of specific binding of [(3)H]glibenclamide to the beef heart mitochondrial membrane. Similar effects were observed with membrane permeable (N-ethylmaleimide) and non-permeable (mersalyl) thiol modifying agents. Glibenclamide binding was also decreased by oxidizing agents (hydrogen peroxide) but not by reducing agents (reduced gluthatione, dithiothreitol and the 2,3-dihydroxy-1,4-dithiolbutane). The results suggest that intact thiol groups, facing the mitochondrial matrix, are essential for glibenclamide binding to the mitochondrial sulfonylurea receptor.<br /> (Copyright 1999 Academic Press.)
- Subjects :
- Adenosine Diphosphate pharmacology
Adenosine Triphosphate pharmacology
Animals
Binding Sites drug effects
Cattle
Dithionitrobenzoic Acid pharmacology
Dose-Response Relationship, Drug
Ethylmaleimide metabolism
Ethylmaleimide pharmacology
Glyburide metabolism
Hydrogen Peroxide pharmacology
Intracellular Membranes drug effects
Intracellular Membranes metabolism
Kinetics
Mersalyl pharmacology
Mitochondria, Heart metabolism
Oxidants pharmacology
Permeability
Reducing Agents pharmacology
Sulfhydryl Compounds metabolism
Sulfonylurea Receptors
ATP-Binding Cassette Transporters
Mitochondria, Heart drug effects
Potassium Channels metabolism
Potassium Channels, Inwardly Rectifying
Receptors, Drug metabolism
Sulfhydryl Reagents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 262
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 10448101
- Full Text :
- https://doi.org/10.1006/bbrc.1999.1190