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Genotoxic activity of chlorohydroxyfuranones in the microscale micronucleus test on mouse lymphoma cells and the unscheduled DNA synthesis assay in rat hepatocytes.
- Source :
-
Mutagenesis [Mutagenesis] 1999 Sep; Vol. 14 (5), pp. 457-62. - Publication Year :
- 1999
-
Abstract
- Chlorohydroxyfuranones (CHFs) are mutagenic disinfection by-products found in chlorine-treated drinking water. In the current study, the genotoxicity of four CHFs, 3,4-dichloro-5-hydroxy-2(5H)-furanone (MCA), 3-chloro-4-methyl-5-hydroxy-2(5H)-furanone (MCF), 3-chloro-4-(chloromethyl)-5-hydroxy-2(5H)-furanone (CMCF) and 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), was determined. Two in vitro assays, the microscale micronucleus assay on L5178Y mouse lymphoma cells and the unscheduled DNA synthesis assay on a hepatocyte primary culture from Fisher F344 rats, were carried out. All four CHFs demonstrated genotoxic effects in both assays. In the two systems used, CMCF was the most genotoxic compound, followed by MCA, MX and MCF, respectively. This work was the first study of the DNA damaging properties of all four CHFs in two in vitro genotoxicity tests. These new data expand the range of genetic damages induced by this group of compounds.
- Subjects :
- Animals
Cell Survival drug effects
DNA biosynthesis
Dose-Response Relationship, Drug
Liver cytology
Liver metabolism
Lymphoma pathology
Male
Mice
Micronucleus Tests
Mutagenicity Tests
Rats
Rats, Inbred F344
Tumor Cells, Cultured
DNA drug effects
DNA Fragmentation drug effects
Furans toxicity
Liver drug effects
Mutagens toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0267-8357
- Volume :
- 14
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Mutagenesis
- Publication Type :
- Academic Journal
- Accession number :
- 10473648
- Full Text :
- https://doi.org/10.1093/mutage/14.5.457