Back to Search
Start Over
Exocytotic insertion of calcium channels constrains compensatory endocytosis to sites of exocytosis.
- Source :
-
The Journal of cell biology [J Cell Biol] 2000 Feb 21; Vol. 148 (4), pp. 755-67. - Publication Year :
- 2000
-
Abstract
- Proteins inserted into the cell surface by exocytosis are thought to be retrieved by compensatory endocytosis, suggesting that retrieval requires granule proteins. In sea urchin eggs, calcium influx through P-type calcium channels is required for retrieval, and the large size of sea urchin secretory granules permits the direct observation of retrieval. Here we demonstrate that retrieval is limited to sites of prior exocytosis. We tested whether channel distribution can account for the localization of retrieval at exocytotic sites. We find that P-channels reside on secretory granules before fertilization, and are translocated to the egg surface by exocytosis. Our study provides strong evidence that the transitory insertion of P-type calcium channels in the surface membrane plays an obligatory role in the mechanism coupling exocytosis and compensatory endocytosis.
- Subjects :
- Animals
Binding Sites drug effects
Cadmium pharmacology
Calcimycin pharmacology
Calcium metabolism
Calcium pharmacology
Calcium Channel Blockers metabolism
Calcium Channel Blockers pharmacology
Calcium Channels, P-Type analysis
Cell Membrane chemistry
Cell Membrane drug effects
Cell Membrane metabolism
Cell Membrane ultrastructure
Concanavalin A metabolism
Cytoplasmic Granules chemistry
Cytoplasmic Granules drug effects
Cytoplasmic Granules ultrastructure
Fertilization physiology
Fluorescent Dyes metabolism
Membrane Potentials drug effects
Membrane Potentials physiology
Microscopy, Electron
Ovum cytology
Ovum drug effects
Ovum ultrastructure
Sea Urchins
Calcium Channels, P-Type metabolism
Cytoplasmic Granules metabolism
Endocytosis drug effects
Exocytosis drug effects
Ovum metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 148
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 10684256
- Full Text :
- https://doi.org/10.1083/jcb.148.4.755