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Vgamma1+ gammadelta T cells play protective roles at an early phase of murine cytomegalovirus infection through production of interferon-gamma.
- Source :
-
Immunology [Immunology] 2000 Feb; Vol. 99 (2), pp. 187-94. - Publication Year :
- 2000
-
Abstract
- Cytomegalovirus (CMV) causes severe opportunistic infection in immunocompromised hosts. The importance of conventional alphabeta T cells in protection against CMV infection has been well documented. However, the role of the second T-cell population (which express the gammadelta T-cell receptor) in CMV infection is not known. In the present study, we analysed the function and protective role of gammadelta T cells in a murine cytomegalovirus (MCMV) infection model. After intraperitoneal infection with MCMV, the number of gammadelta T cells increased in the liver and peritoneal cavity from day 3, and reached a peak on day 5. The gammadelta T cells showed an activated T-cell phenotype and predominantly expressed Vgamma1, which is known to be expressed by heat-shock protein 65 (hsp 65)-specific gammadelta T cells. Analysis of cytokine expression demonstrated that the MCMV-induced gammadelta T cells expressed interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) but not interleukin-4 (IL-4), implying their participation in the cell-mediated immune response against MCMV. Depletion of gammadelta T cells by anti-T-cell receptor (TCR) gammadelta monoclonal antibody (mAb) treatment resulted in significant increase of virus titre and decrease of IFN-gamma in the liver on day 3 after MCMV infection, which further supports the importance of gammadelta T cells in early protection against infection. Finally, the MCMV-induced gammadelta T cells produced IFN-gamma in vitro in response to hsp 65. Our results suggest that gammadelta T cells participate in early protection against MCMV infection through recognition of hsp 65 and production of IFN-gamma.
- Subjects :
- Animals
Chaperonin 60
Chaperonins immunology
Cytokines biosynthesis
Female
Interleukin-12 immunology
Liver immunology
Liver virology
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Peritoneal Cavity pathology
Virus Replication immunology
Bacterial Proteins
Herpesviridae Infections immunology
Interferon-gamma biosynthesis
Muromegalovirus growth & development
Receptors, Antigen, T-Cell, gamma-delta analysis
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-2805
- Volume :
- 99
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 10692035
- Full Text :
- https://doi.org/10.1046/j.1365-2567.2000.00938.x