Intratracheal exposure of the guinea pig lung to cadmium and/or selenium: a histological evaluation.

Anesthetized male Hartley guinea pigs (350-410 g) (n=5) received intratracheally, saline; cadmium (Cd) (0.3 mg); selenium (Se) (0.3 or 0.06 mg); or Cd (0.3 mg) with Se (0.06 mg), per animal. Twenty-four hours later, lungs were evaluated. Bronchoalveolar-lavage fluid of Cd- and/or Se-treated animals varied in their total and differential leukocyte percent population from that of saline control (P<0.05). Cadmium alone or with Se caused high lung to body weight ratios (P<0.05). High lung wet-weight to dry-weight (W/D) ratios (P<0.05) suggestive of lung edema, were evident after Cd and/or Se exposure. Histological examination of Cd- and/or Se-exposed lungs revealed leukocytic infiltration. Results demonstrated that separate or concurrent exposure to noxious metal(s) such as Cd and Se provoke lung edema and injury. Low dose of Se which when instilled alone, although did not result in an increased W/D lung ratio, failed to subside concurrently administered Cd-inflicted damage.