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Polynucleotides. XXVI. Complex formation of polynucleotides derived from formycin and laurusin with cyclonucleoside oligonucleotides.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1974 Jul; Vol. 1 (7), pp. 907-17. - Publication Year :
- 1974
-
Abstract
- Poly(formycin phosphate) and poly(laurusin phosphate) were synthesized by polymerizing formycin and laurusin 5'-diphosphate by means of E. coli polynucleotide phosphorylase. The complex formation of these polynucleotides with cyclonucleoside polynucleotides were investigated. While poly(formycin phosphate) did not form the complex with an octanucleotide of 6,2'-anhydro-6-oxy-1-beta-D-arabinofuranosyluracil, poly(laurusin phosphate) did form a 1: 1 complex with octanucleotide of 8,2'-anhydro-8-mercapto-9-beta-D-arabinofuranosyladenine in the presence of 0.15M Na ion at neutrality and 3(o). CD spectrum of this complex showed a couple of a trough at 286 nm and a peak at 262 nm. This fact suggests that the complex has a left-handed helical conformation, which is opposite to the natural double helical polynucleotides. The cause of this phenomenon was discussed in connection with the complex of cyclonucleoside oligonucleotides.
- Subjects :
- Circular Dichroism
Escherichia coli enzymology
Formycins chemistry
Nucleic Acid Conformation
Nucleotides, Cyclic metabolism
Oligonucleotides metabolism
Polynucleotides chemistry
Polyribonucleotide Nucleotidyltransferase metabolism
Spectrophotometry, Ultraviolet
Formycins metabolism
Polynucleotides biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 1
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 10793723
- Full Text :
- https://doi.org/10.1093/nar/1.7.907