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Retinoblastoma susceptibility protein, Rb, possesses multiple BRCT-Ws, BRCA1 carboxyl-terminus-related W regions with DNA break-binding activity.
- Source :
-
Oncogene [Oncogene] 2000 Apr 13; Vol. 19 (16), pp. 1982-91. - Publication Year :
- 2000
-
Abstract
- The BRCT region, the carboxyl-terminus of BRCA1 (the breast cancer susceptibility gene 1 product), is ubiquitous in several proteins that participate in cell cycle checkpoints and DNA repair. We have previously shown that the BRCT regions of TopBP1 (DNA topoisomerase II binding protein 1) and BRCA1 bound DNA breaks. A BRCT-related region, BRCT-W1, in the retinoblastoma susceptibility gene product (Rb) also could bind DNA fragments, independently of DNA sequences. Five BRCT-W regions were found in the Rb family. All BRCT-Ws of Rb bound DNA fragments. Electron microscopy and treatment with an exonuclease showed that BRCT-Ws bound double-strand DNA breaks. Since some BRCTs are exceptional common relating elements in tumor suppression, our findings reveal novel aspects of the tumor suppression mechanism.
- Subjects :
- Amino Acid Sequence
Antigens, Viral, Tumor metabolism
Carrier Proteins metabolism
DNA chemistry
DNA metabolism
DNA Damage
DNA-Binding Proteins
Endodeoxyribonucleases metabolism
Escherichia coli genetics
Microscopy, Electron
Molecular Sequence Data
Nuclear Proteins
Peptide Fragments metabolism
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Retinoblastoma Protein chemistry
Retinoblastoma Protein genetics
Sequence Homology, Amino Acid
BRCA1 Protein metabolism
Retinoblastoma Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0950-9232
- Volume :
- 19
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 10803459
- Full Text :
- https://doi.org/10.1038/sj.onc.1203528