Back to Search Start Over

Efficient induction of peptide-specific cytotoxic T lymphocytes by LPS-activated spleen cells.

Authors :
Kakugawa K
Udaka K
Nakashima K
Inaba K
Oka Y
Sugiyama H
Tamamura H
Yamagishi H
Source :
Microbiology and immunology [Microbiol Immunol] 2000; Vol. 44 (2), pp. 123-33.
Publication Year :
2000

Abstract

Lipopolysaccharides of gram-negative bacteria are potent activators of B cells, dendritic cells and monocytes/macrophages. We have investigated the use of LPS-activated spleen cells as antigen-presenting cells to induce CD8+ cytotoxic T lymphocytes in vivo that are reactive to MHC class I binding peptides. Compared with resting spleen cells, CTL induction was more efficient and less variable for different peptides with LPS-activated spleen cells. Cytotoxic responses were specific for the immunized peptides and contained high affinity CD8+ T cells. The removal of dendritic cells and monocytes/macrophages by Sephadex G10 column did not show profound effects on CTL induction, indicating that B-cell blasts were largely responsible. This easily accessible method should facilitate the screening of MHC class I binding peptides to determine whether or not the host's T-cell repertoire contains reactive T cells.

Details

Language :
English
ISSN :
0385-5600
Volume :
44
Issue :
2
Database :
MEDLINE
Journal :
Microbiology and immunology
Publication Type :
Academic Journal
Accession number :
10803499
Full Text :
https://doi.org/10.1111/j.1348-0421.2000.tb01255.x