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Blockade of L-selectin attenuates reperfusion injury in a rat model.
- Source :
-
Journal of reconstructive microsurgery [J Reconstr Microsurg] 2000 Apr; Vol. 16 (3), pp. 227-33. - Publication Year :
- 2000
-
Abstract
- Ischemia/reperfusion (I/R) injury appears to be a significant neutrophil-dependent component and may be ameliorated by blocking leukocyte-endothelial adhesion. Using a rat extensor digitorum longus (EDL) muscle model, the present study tested the hypothesis that in vivo administration of the function-blocking monoclonal antibody (mAb) LAM1-116 which recognizes L-selectin, a cell-surface adhesion receptor, could decrease I/R injury. In 46 rats, one EDL served as a normal control and the opposite EDL underwent 3 hr of ischemia followed by 3 hr of reperfusion after pretreatment with LAM1-116 mAb, control IgG, or saline. Myeloperoxidase (MPO) activity showed only a two-fold increase from normal in LAM1-116-treated I/R EDL while a 27-fold increase occurred in the IgG2a and saline groups, with a statistically significant (p < 0.001) difference. A significantly (p < 0.05) lower wet weight ratio, improved fatigue contractile force, and less neutrophil infiltration were found in LAM1-116-treated EDL, when compared to those in control IgG- or saline-treated EDL. The results indicate that blockade of L-selectin by LAM1-116 mAb can effectively reduce neutrophil infiltration in reperfused skeletal muscle, thereby decreasing tissue edema and improving muscle fatigue contractile force. These findings may be important in understanding I/R injury.
- Subjects :
- Animals
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Immunoglobulin G metabolism
Immunoglobulin G pharmacology
L-Selectin metabolism
Leukocyte Count drug effects
Muscle Contraction drug effects
Muscle, Skeletal pathology
Organ Size drug effects
Peroxidase analysis
Rats
Rats, Sprague-Dawley
Reference Values
Reperfusion Injury physiopathology
Sensitivity and Specificity
Antibodies, Monoclonal pharmacology
L-Selectin drug effects
Peroxidase metabolism
Reperfusion Injury drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 0743-684X
- Volume :
- 16
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of reconstructive microsurgery
- Publication Type :
- Academic Journal
- Accession number :
- 10803628
- Full Text :
- https://doi.org/10.1055/s-2000-7557