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Molecular cloning and characterization of phosphatidylcholine transfer protein-like protein gene expressed in murine haploid germ cells.
- Source :
-
Biology of reproduction [Biol Reprod] 2000 Jun; Vol. 62 (6), pp. 1694-1701. - Publication Year :
- 2000
-
Abstract
- We have isolated a cDNA clone specifically expressed in spermiogenesis from a subtracted cDNA library of mouse testis. The cDNA consisted of 1392 nucleotides and had an open reading frame of 873 nucleotides encoding a protein of 291 amino acid residues. Computer-mediated homology search revealed that the nucleotide sequence was unique but the deduced amino acid sequence had similarity to mouse phosphatidylcholine transfer protein (PCTP). We named this newly isolated gene PCTP-like protein. Northern blot analysis revealed a 1.4-kilobase mRNA expressed in the testis, kidney, liver, and intestine with the highest level in the testis. Messenger RNA expression in the testis was detected first on Day 23 in postnatal development and then increased up to adulthood. The protein, having a molecular weight of approximately 40 000, was encoded by the mRNA and was detected at the tail of the elongated spermatids and sperm by immunohistochemical staining.
- Subjects :
- Aging
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Blotting, Western
Carrier Proteins chemistry
Carrier Proteins genetics
DNA, Complementary chemistry
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Phospholipid Transfer Proteins
RNA, Messenger analysis
Sequence Homology
Sperm Tail chemistry
Spermatids chemistry
Testis chemistry
Androgen-Binding Protein
Cloning, Molecular
Gene Expression
Haploidy
Spermatozoa chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3363
- Volume :
- 62
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biology of reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 10819773
- Full Text :
- https://doi.org/10.1095/biolreprod62.6.1694