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Inducible expression of a p56Lck transgene reveals a central role for Lck in the differentiation of CD4 SP thymocytes.
- Source :
-
Immunity [Immunity] 2000 May; Vol. 12 (5), pp. 537-46. - Publication Year :
- 2000
-
Abstract
- The T lymphocyte-specific protein tyrosine kinase p56lck (Lck) is an essential component of the TCR-mediated signal transduction complex. Lck knockout mice have reduced numbers of double-positive thymocytes and very few mature single-positive cells, particularly of the CD4 lineage. Here we demonstrate the ability of a tetracycline-based tissue-specific inducible Lck transgene to restore expansion of early thymocytes and maturation of single-positive cells in Lckneg mice upon induction with doxycycline. Restoration of Lck expression is particularly important for positive selection to the CD4+ lineage but has a lesser impact on selection to the CD8+ lineage, suggesting activation of Lck is an important component of the signals involved in lineage choice during thymic differentiation.
- Subjects :
- Animals
CD4 Antigens
Cell Differentiation immunology
Gene Expression Regulation immunology
Lymphocyte Specific Protein Tyrosine Kinase p56(lck) genetics
Mice
Mice, Knockout
Mice, Transgenic
CD4-Positive T-Lymphocytes immunology
Cell Lineage immunology
Lymphocyte Specific Protein Tyrosine Kinase p56(lck) immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1074-7613
- Volume :
- 12
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Immunity
- Publication Type :
- Academic Journal
- Accession number :
- 10843386
- Full Text :
- https://doi.org/10.1016/s1074-7613(00)80205-8