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Expression of retinoid receptors during the retinoic acid-induced neuronal differentiation of human embryonal carcinoma cells.
- Source :
-
Journal of neurochemistry [J Neurochem] 2000 Jul; Vol. 75 (1), pp. 34-40. - Publication Year :
- 2000
-
Abstract
- Retinoic acid (RA), a derivative of vitamin A, is essential for normal patterning and neurogenesis during development. Until recently, studies have been focused on the physiological roles of RA receptors (RARs), one of the two types of nuclear receptors, whereas the functions of the other nuclear receptors, retinoid X receptors (RXRs), have not been explored. Accumulating evidence now suggests that RXRalpha is a critical receptor component mediating the effects of RA during embryonic development. In this study, we have examined the expression profiles of RXRalpha and RARs during the RA-induced neuronal differentiation in a human embryonal carcinoma cell line, NT2. Distinct expression profiles of RXRalpha, RARalpha, RARbeta, and RARgamma were observed following treatment with RA. In particular, we found that RA treatment resulted in a biphasic up-regulation of RXRalpha expression in NT2 cells. The induced RXRalpha was found to bind specifically to the retinoid X response element based on gel mobility retardation assays. Furthermore, immunocytochemical analysis revealed that RXRalpha expression could be localized to the somatoaxonal regions of the NT2 neurons, including the tyrosine hydroxylase- and vasoactive intestinal peptide-positive neurons. Taken together, our findings provide the first demonstration of the cellular localization and regulation of RXRalpha expression in NT2 cells and suggest that RXRalpha might play a crucial role in the cellular functions of human CNS neurons.
- Subjects :
- DNA metabolism
Embryonal Carcinoma Stem Cells
Humans
Neurons ultrastructure
RNA, Messenger analysis
Receptors, Retinoic Acid metabolism
Response Elements
Retinoid X Receptors
Subcellular Fractions chemistry
Transcription Factors genetics
Transcription Factors metabolism
Tumor Cells, Cultured
Cell Differentiation drug effects
Gene Expression
Neoplastic Stem Cells drug effects
Neurons drug effects
Receptors, Retinoic Acid genetics
Tretinoin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 75
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 10854244
- Full Text :
- https://doi.org/10.1046/j.1471-4159.2000.0750034.x