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Xplore mRNA assays for the quantification of IL-1 beta and TNF-alpha mRNA in lipopolysaccharide-induced mouse macrophages.
- Source :
-
BioTechniques [Biotechniques] 2000 Jun; Vol. 28 (6), pp. 1220-1, 1224-5. - Publication Year :
- 2000
-
Abstract
- Because the accurate measurement of a number of cytokine mRNA transcripts provides valuable knowledge about cytokine gene regulation, we have developed the Xplore assay for the quantification of cytokine mRNA. This microplate-based assay is rapid (under four hours), quantitative over three orders of magnitude and carries no risk of false-positive values from contamination with amplified target. Here, we describe the use of Xplore assays to measure the steady-state mRNA levels of TNF-alpha and IL-1 beta produced by mouse WEHI and J774 macrophage-like cell lines.
Details
- Language :
- English
- ISSN :
- 0736-6205
- Volume :
- 28
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- BioTechniques
- Publication Type :
- Academic Journal
- Accession number :
- 10868288
- Full Text :
- https://doi.org/10.2144/00286pf01