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Functional significance of prop-1 gene expression in pituitary adenomas.
- Source :
-
Endocrine journal [Endocr J] 2000 Mar; Vol. 47 Suppl, pp. S85-9. - Publication Year :
- 2000
-
Abstract
- Prophet of Pit-1 (Prop-1), which is a paired-like homeodomain transcription factor, is capable of binding to sites in an early enhancer of the Pit-1 gene and regulating its expression. As human Pit-1 is expressed considerably in pituitary adenomas, we studied human Prop-1 gene expression in pituitary adenomas. We also sequenced the Prop-1 cDNAs in pituitary adenomas. Human Prop-1 transcript was detected in all pituitary adenomas examined by RT-PCR analysis. The expression of human Prop-1 in pituitary adenomas was confirmed by in situ hybridization in one of the GH-producing adenomas. The sequence analysis of human Prop-1 cDNAs in these pituitary adenomas revealed that there were no mutations except 5 silent nucleic acid substitutions, suggesting that mutations of Prop-1 gene do not represent a frequent mechanism of human pituitary tumorigenesis.
- Subjects :
- Adenoma pathology
Base Sequence genetics
Humans
In Situ Hybridization
Pituitary Neoplasms pathology
Reverse Transcriptase Polymerase Chain Reaction
Adenoma genetics
Adenoma physiopathology
Gene Expression
Homeodomain Proteins genetics
Pituitary Neoplasms genetics
Pituitary Neoplasms physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 0918-8959
- Volume :
- 47 Suppl
- Database :
- MEDLINE
- Journal :
- Endocrine journal
- Publication Type :
- Academic Journal
- Accession number :
- 10890192
- Full Text :
- https://doi.org/10.1507/endocrj.47.supplmarch_s85