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Binding of a large chondroitin sulfate/dermatan sulfate proteoglycan, versican, to L-selectin, P-selectin, and CD44.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2000 Nov 10; Vol. 275 (45), pp. 35448-56. - Publication Year :
- 2000
-
Abstract
- Here we show that a large chondroitin sulfate proteoglycan, versican, derived from a renal adenocarcinoma cell line ACHN, binds L-selectin, P-selectin, and CD44. The binding was mediated by the interaction of the chondroitin sulfate (CS) chain of versican with the carbohydrate-binding domain of L- and P-selectin and CD44. The binding of versican to L- and P-selectin was inhibited by CS B, CS E, and heparan sulfate (HS) but not by any other glycosaminoglycans tested. On the other hand, the binding to CD44 was inhibited by hyaluronic acid, chondroitin (CH), CS A, CS B, CS C, CS D, and CS E but not by HS or keratan sulfate. A cross-blocking study indicated that L- and P-selectin recognize close or overlapping sites on versican, whereas CD44 recognizes separate sites. We also show that soluble L- and P-selectin directly bind to immobilized CS B, CS E, and HS and that soluble CD44 directly binds to immobilized hyaluronic acid, CH, and all the CS chains examined. Consistent with these results, structural analysis showed that versican is modified with at least CS B and CS C. Thus, proteoglycans sufficiently modified with the appropriate glycosaminoglycans should be able to bind L-selectin, P-selectin, and/or CD44.
- Subjects :
- Animals
Biotinylation
Blotting, Western
Chondroitin pharmacology
Chondroitin ABC Lyase pharmacology
Chondroitinases and Chondroitin Lyases pharmacology
Cross-Linking Reagents pharmacology
DNA, Complementary metabolism
Dose-Response Relationship, Drug
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Glycosaminoglycans metabolism
Humans
Hyaluronic Acid pharmacology
Keratan Sulfate pharmacology
Kinetics
Lectins, C-Type
Lipid Metabolism
Mice
Protein Binding
Transfection
Tumor Cells, Cultured
Versicans
Chondroitin Sulfate Proteoglycans metabolism
Chondroitin Sulfates metabolism
Dermatan Sulfate metabolism
Hyaluronan Receptors metabolism
L-Selectin metabolism
P-Selectin metabolism
Proteoglycans metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 275
- Issue :
- 45
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 10950950
- Full Text :
- https://doi.org/10.1074/jbc.M003387200