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Conservation of heterochromatin protein 1 function.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 2000 Sep; Vol. 20 (18), pp. 6970-83. - Publication Year :
- 2000
-
Abstract
- Heterochromatin represents a cytologically visible state of heritable gene repression. In the yeast, Schizosaccharomyces pombe, the swi6 gene encodes a heterochromatin protein 1 (HP1)-like chromodomain protein that localizes to heterochromatin domains, including the centromeres, telomeres, and the donor mating-type loci, and is involved in silencing at these loci. We identify here the functional domains of swi6p and demonstrate that the chromodomain from a mammalian HP1-like protein, M31, can functionally replace that of swi6p, showing that chromodomain function is conserved from yeasts to humans. Site-directed mutagenesis, based on a modeled three-dimensional structure of the swi6p chromodomain, shows that the hydrophobic amino acids which lie in the core of the structure are critical for biological function. Gel filtration, gel overlay experiments, and mass spectroscopy show that HP1 proteins can self-associate, and we suggest that it is as oligomers that HP1 proteins are incorporated into heterochromatin complexes that silence gene activity.
- Subjects :
- Amino Acid Sequence
Animals
Binding Sites
Chromosomal Proteins, Non-Histone chemistry
Chromosomal Proteins, Non-Histone genetics
Chromosomes, Fungal
Female
Fungal Proteins chemistry
Fungal Proteins genetics
Humans
Molecular Sequence Data
Mutagenesis, Site-Directed
Nuclear Localization Signals
Protein Structure, Quaternary
Rats
Rats, Inbred F344
Schizosaccharomyces genetics
Transcription Factors chemistry
Transcription Factors genetics
Chromosomal Proteins, Non-Histone physiology
Evolution, Molecular
Fungal Proteins physiology
Heterochromatin physiology
Saccharomyces cerevisiae Proteins
Schizosaccharomyces physiology
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0270-7306
- Volume :
- 20
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 10958692
- Full Text :
- https://doi.org/10.1128/MCB.20.18.6970-6983.2000