Back to Search
Start Over
Nitrated proteins in bronchoalveolar lavage fluid of patients at risk of ventilator-associated bronchopneumonia.
- Source :
-
The European respiratory journal [Eur Respir J] 2000 Aug; Vol. 16 (2), pp. 296-301. - Publication Year :
- 2000
-
Abstract
- The study was designed to identify markers of oxidative injury, related to the nitric oxide derived cascade, in bronchoalveolar lavage (BAL) fluid from intensive care patients suspected of ventilator-associated pneumonia (VAP) and/or acute respiratory distress syndrome (ARDS). Thirty-eight patients developing VAP and/or ARDS (VAP/ARDS group) were compared to 20 ventilated patients without VAP/ARDS (control group). Myeloperoxidase (MPO) and elastase, taken as markers of neutrophil activation were measured by enzymatic techniques, and nitrated proteins (NTPs) by an immunological method. The cytotoxicity of the BAL fluid was tested using cultured human epithelial alveolar cells by the release of pre-incorporated 51Cr. Mean NTP concentration and, MPO and elastase activities were different between the VAP/ARDS and control groups (p<0.05 for NTPs; p<0.005 for MPO; p<0.005 for elastase). NTP concentration correlated with MPO and elastase activity and neutrophil number (r=0.93, 0.91 and 0.87, respectively), but not to protein concentration and arterial oxygen tension/inspiratory oxygen fraction. The cytotoxicity of BAL correlated with NTP concentration (r=0.92) and MPO activity (r=0.89). It was concluded that the concentrations of nitrated proteins in bronchoalveolar lavage fluid correlated with the oxidant activity of neutrophils and that, bronchoalveolar lavage fluid cytotoxicity was correlated with the nitrated protein concentration and may be mediated by oxidants.
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Cell Survival
Cells, Cultured
Epithelial Cells physiology
Female
Humans
Male
Middle Aged
Neutrophils enzymology
Pancreatic Elastase metabolism
Peroxidase metabolism
Pulmonary Alveoli cytology
Pulmonary Alveoli physiology
Reference Values
Respiratory Distress Syndrome metabolism
Risk Factors
Bronchoalveolar Lavage Fluid chemistry
Bronchopneumonia etiology
Bronchopneumonia metabolism
Neutrophils physiology
Nitrates metabolism
Proteins metabolism
Respiration, Artificial adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 0903-1936
- Volume :
- 16
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The European respiratory journal
- Publication Type :
- Academic Journal
- Accession number :
- 10968506
- Full Text :
- https://doi.org/10.1034/j.1399-3003.2000.16b18.x