Back to Search
Start Over
Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells.
- Source :
-
Journal of neural transmission (Vienna, Austria : 1996) [J Neural Transm (Vienna)] 2000; Vol. 107 (8-9), pp. 919-29. - Publication Year :
- 2000
-
Abstract
- This study was conducted to investigate an effect of heat stress at 44 degrees C for 30 min on intracellular Ca2+ signaling system and on heat shock protein (HSP)-70 expression. 5-HT-induced Ca2+ mobilization was reduced 1, 3 and 6 hrs after heat stress, and recovered to the control level 12 and 24 hrs after heat stress. One hr after heat stress, Ca2+ rise was significantly decreased when the cells were stimulated by any concentration of 5-HT. Thrombin-induced Ca2+ increase was also markedly reduced 1 hr after heat stress. HSP-70 level was increased 6 and 9 hr after heat stress. In HSP synthesis inhibitor quercetin-treated cells, HSP-70 expression was not enhanced after heat stress, and Ca2+ rise in response to 5-HT did not return to the control level. However, the Ca2+ rise induced by 5-HT was not restored to the control level after stress in Ac-Asp-Glu-Val-Asp-H (DEVD)-exposed cells while DEVD had little effect on heat stress-induced synthesis of HSP-70. Dexamethasone did not alter the change in HSP-70 expression or Ca2+ response after heat stress. These results indicate that heat stress attenuated 5-HT-induced Ca2+ mobilization and that HSP-70 expression played an important role in recovery from Ca2+ impairment, possibly via protease activity in C6 cells.
- Subjects :
- Animals
Calcium Signaling drug effects
Calcium Signaling physiology
Cysteine Proteinase Inhibitors pharmacology
Dexamethasone pharmacology
Glucocorticoids pharmacology
HSP70 Heat-Shock Proteins metabolism
Heat-Shock Response drug effects
Hot Temperature
Oligopeptides pharmacology
Quercetin pharmacology
Rats
Receptor, Serotonin, 5-HT2A
Serotonin metabolism
Tumor Cells, Cultured
Calcium metabolism
Glioma
Heat-Shock Response physiology
Receptors, Serotonin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0300-9564
- Volume :
- 107
- Issue :
- 8-9
- Database :
- MEDLINE
- Journal :
- Journal of neural transmission (Vienna, Austria : 1996)
- Publication Type :
- Academic Journal
- Accession number :
- 11041272
- Full Text :
- https://doi.org/10.1007/s007020070042