Back to Search
Start Over
Developmental expression of muscarinic acetylcholine receptors in chick retina: selective induction of M2 muscarinic receptor expression in ovo by a factor secreted by muller glial cells.
- Source :
-
The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2000 Nov 15; Vol. 20 (22), pp. 8417-25. - Publication Year :
- 2000
-
Abstract
- Muscarinic acetylcholine receptors (mAChRs) play an important role in signal processing in the retina. We have used subtype-specific antibodies to identify the changes in the localization of mAChR expression during embryonic development of the retina in vivo and their relationship to the changes in mAChRs in retinal cells in culture. We have demonstrated previously that treatment of fresh retinal cultures with conditioned media from mature retinal cultures specifically induces expression of the M(2) mAChR (McKinnon et al., 1998). We show that the M(2)-inducing activity, which we tentatively have called MARIA (muscarinic acetylcholine receptor-inducing activity) is produced by Müller glial cells in culture, because significant activity can be found in media conditioned by essentially neuron-free cultures of Müller glia, as well as by a Müller glial cell line but not several neuroblastoma cell lines. We also demonstrate that the appearance of the M(2) receptor in vivo occurs concomitantly with the appearance of significant numbers of Müller glial cells in the developing retina. Furthermore, the administration of crude or partially purified preparations of MARIA to developing chick embryos in ovo induces precocious expression of M(2) mAChRs in the appropriate cell types in the retina. These results show that a factor secreted by cultured retinal Müller glia can regulate M(2) mAChR expression in vivo and in vitro and suggest that the secretion of MARIA by Müller glia in vivo may be responsible for the normal induction of M(2) mAChR expression during embryonic development.
- Subjects :
- Animals
Biological Factors isolation & purification
Biological Factors pharmacology
Cells, Cultured
Chick Embryo
Culture Media, Conditioned chemistry
Culture Media, Conditioned metabolism
Culture Media, Conditioned pharmacology
Gene Expression Regulation, Developmental drug effects
Immunohistochemistry
Neuroglia cytology
Ovum cytology
Receptor, Muscarinic M2
Receptors, Muscarinic genetics
Retina cytology
Tenascin metabolism
Biological Factors metabolism
Gene Expression Regulation, Developmental physiology
Neuroglia metabolism
Ovum metabolism
Receptors, Muscarinic biosynthesis
Retina embryology
Retina metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1529-2401
- Volume :
- 20
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 11069949