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Pyrokinin neuropeptides in a crustacean. Isolation and identification in the white shrimp Penaeus vannamei.
- Source :
-
European journal of biochemistry [Eur J Biochem] 2001 Jan; Vol. 268 (1), pp. 149-54. - Publication Year :
- 2001
-
Abstract
- Identification of substances able to elicit physiological or behavioural processes that are related to reproduction would greatly contribute to the domestication of commercially important crustaceans that do not reproduce easily in captivity. Crustaceans are thought to release urine signals used for chemical communication involved in courtship behaviour. In contrast to insects, very little is known about the endocrinological processes underlying this phenomenon. Therefore, an extract of 3500 central nervous systems of female white shrimp Penaeus vannamei was screened for myotropic activity in order to purify pyrokinin-like peptides that belong to the pyrokinin/PBAN neuropeptide family. Members of this family regulate reproductive processes in insects, including pheromone biosynthesis. Purification of these pyrokinins was achieved by a combination of reversed-phase and normal-phase chromatography. Subsequent characterization by mass spectrometry, Edman degradation and peptide synthesis resulted in the elucidation of two novel peptides. Pev-PK 1 has the primary sequence DFAFSPRL-NH(2) and a second peptide (Pev-PK 2) is characterized as the nonapeptide ADFAFNPRL-NH(2). Pev-PK 1 contains the typical FXPRL-NH(2) (X = G, S, T or V) C-terminal sequence that characterizes members of the versatile pyrokinin/PBAN family. Pev-PK 2 displays an Asn residue at the variable X position of the core pyrokinin sequence. These crustacean pyrokinins are the first to be found in a noninsect. The synthetic peptides display myotropic activity on the Leucophaea maderae as well as on the Astacus leptodactylus hindgut.
- Subjects :
- Amino Acid Sequence
Animals
Chromatography, High Pressure Liquid
Molecular Sequence Data
Neuropeptides chemistry
Neuropeptides physiology
Sequence Homology, Amino Acid
Shellfish
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Central Nervous System chemistry
Neuropeptides isolation & purification
Penaeidae chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2956
- Volume :
- 268
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 11121115
- Full Text :
- https://doi.org/10.1046/j.1432-1327.2001.01858.x