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Preparation of human-mouse heterohybridomas against an immunising antigen.
- Source :
-
Journal of immunological methods [J Immunol Methods] 2000 Dec 01; Vol. 246 (1-2), pp. 187-202. - Publication Year :
- 2000
-
Abstract
- The production of murine monoclonal antibodies against specific antigens by hybridomas is a well utilised technique. The production of hybridomas secreting specific human antibodies would have many advantages in therapeutic applications of monoclonal antibodies. The immortalised human lymphocytes themselves would also provide valuable tools in research on lymphocyte development. Preparation of human-human hybridomas has been limited by a lack of suitable fusion partners. This protocol paper describes the production of human-mouse heterohybridomas by two independent laboratories. The purpose of this protocol is to provide a basis for the development of heterohybridoma technology in laboratories with limited hybridoma experience.
- Subjects :
- Adult
Animals
Antibodies, Monoclonal biosynthesis
Antibodies, Monoclonal immunology
Cell Division physiology
Cell Fusion methods
Culture Media
Humans
Hybridomas cytology
Hybridomas metabolism
Infant
Lymphocytes cytology
Lymphocytes immunology
Mice
Multiple Myeloma immunology
Multiple Myeloma pathology
Polyethylene Glycols
Antigens immunology
Hybridomas immunology
Pneumococcal Vaccines immunology
Tetanus Toxoid immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1759
- Volume :
- 246
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 11121559
- Full Text :
- https://doi.org/10.1016/s0022-1759(00)00293-3