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Expression of apoptosis markers on peripheral blood lymphocytes from patients with cutaneous T-cell lymphoma during extracorporeal photochemotherapy.
- Source :
-
Journal of the American Academy of Dermatology [J Am Acad Dermatol] 2001 Jan; Vol. 44 (1), pp. 40-7. - Publication Year :
- 2001
-
Abstract
- The mechanisms of extracorporeal photochemotherapy (ExP) therapeutic activity in cutaneous T-cell lymphomas (CTCLs) are not yet well understood, even though it has been suggested that a major mechanism may be induction of apoptosis. In vitro studies demonstrate that UVA-induced apoptosis is mediated by CD95-Fas expression and inhibited by Bcl-2 up-regulation and that UVA irradiation is able to down-regulate Bcl-2 expression. High-resolution multiparameter flow-cytometric analyses were used to evaluate Bcl-2/CD95-Fas expression on phenotypically identifiable circulating clonal T cells from 7 patients with CTCL (4 with Sézary syndrome and 3 with mycosis fungoides with peripheral involvement) before and during ExP, in an attempt to ascertain whether Bcl-2/CD95-Fas status can be related to the hematologic response. A Bcl-2 normal phenotype before ExP or a normalization in Bcl-2 expression during ExP were related to a better clinical response, whereas a persistent Bcl-2 high expression was a negative prognostic factor. On the other hand, no response was found in patients with a CD95-Fas-negative phenotype, whereas the expression of CD95-Fas was associated with hematologic remission. Although further studies are needed to confirm these preliminary results, this study suggests that Bcl-2 and CD95-Fas expression could be evaluated, together with the other known clinical and immunologic factors, as additional parameters related to clinical response in patients with CTCL undergoing ExP.
- Subjects :
- Adult
Aged
Down-Regulation
Female
Flow Cytometry
Gene Rearrangement
Genes, T-Cell Receptor
Humans
Male
Middle Aged
Mycosis Fungoides drug therapy
Mycosis Fungoides immunology
Sezary Syndrome drug therapy
Sezary Syndrome immunology
Apoptosis
Biomarkers blood
Lymphocytes chemistry
Lymphoma, T-Cell, Cutaneous drug therapy
Lymphoma, T-Cell, Cutaneous immunology
Photochemotherapy methods
Proto-Oncogene Proteins c-bcl-2 blood
Skin Neoplasms drug therapy
Skin Neoplasms immunology
fas Receptor blood
Subjects
Details
- Language :
- English
- ISSN :
- 0190-9622
- Volume :
- 44
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of the American Academy of Dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 11148475
- Full Text :
- https://doi.org/10.1067/mjd.2001.108376