Back to Search
Start Over
Chemioxyexcitation (delta pO2/ROS)-dependent release of IL-1 beta, IL-6 and TNF-alpha: evidence of cytokines as oxygen-sensitive mediators in the alveolar epithelium.
- Source :
-
Cytokine [Cytokine] 2001 Feb 07; Vol. 13 (3), pp. 138-47. - Publication Year :
- 2001
-
Abstract
- The signalling mechanisms in oxidative stress mediated by cytokines in the perinatal alveolar epithelium are not well known. In an in vitro model of fetal alveolar type II epithelial cells, we investigated the profile of cytokines in response to ascending Deltap O(2)regimen (oxyexcitation). The peak of TNF-alpha (4 h) preceded IL-1beta and IL-6 (6-9 h), indicating a positive feedback autocrine loop confirmed by exogenous rmTNF-alpha. Reactive oxygen species (ROS) induced a dose-dependent release of cytokines, an effect specifically obliterated by selective antioxidants of the hydroxyl radical (*OH) and superoxide anion (O(2)-). Actinomycin and cycloheximide blocked the induced production of cytokines, implicating transcriptional and translational control. Whilst the dismutating enzymes superoxide dismutase (SOD) and catalase were ineffective in reducing ROS-induced cytokines, MnP, a cell-permeating SOD mimetic, abrogated xanthine/xanthine oxidase-dependent cytokine release. Desferrioxamine mesylate, which inhibits the iron-catalysed generation of *OH via the Fenton reaction, exhibited a mild effect on the release of cytokines. Dynamic variation in alveolar p O(2)constitutes a potential signalling mechanism within the perinatal lung allowing upregulation of cytokines in an ROS-dependent manner.<br /> (Copyright 2001 Academic Press.)
- Subjects :
- Animals
Antioxidants pharmacology
Catalase pharmacology
Cells, Cultured
Cycloheximide pharmacology
Cytokines biosynthesis
Cytokines physiology
Dactinomycin pharmacology
Female
Hydrogen Peroxide metabolism
Hydroxyl Radical metabolism
Interleukin-1 physiology
Interleukin-6 physiology
Mice
Oxidative Stress drug effects
Oxidative Stress physiology
Pulmonary Alveoli enzymology
Pulmonary Alveoli metabolism
Rats
Reactive Oxygen Species physiology
Respiratory Mucosa enzymology
Respiratory Mucosa metabolism
Superoxide Dismutase pharmacology
Tumor Necrosis Factor-alpha physiology
Cytokines metabolism
Interleukin-1 metabolism
Interleukin-6 metabolism
Oxygen toxicity
Pulmonary Alveoli physiology
Reactive Oxygen Species metabolism
Respiratory Mucosa physiology
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1043-4666
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cytokine
- Publication Type :
- Academic Journal
- Accession number :
- 11161456
- Full Text :
- https://doi.org/10.1006/cyto.2000.0789