Back to Search
Start Over
Expression of the 17beta-hydroxysteroid dehydrogenase type 5 mRNA in the human brain.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 2001 Jan 22; Vol. 171 (1-2), pp. 165-8. - Publication Year :
- 2001
-
Abstract
- An enzyme-mediated metabolism of androgens and estrogens including 17beta-HSD activity in the brain of vertebrates was discovered approximately 30 years ago. Mainly 5alpha-reductase and aromatase have been studied in detail. Recently we could demonstrate reductive and oxidative 17beta-HSD activity as well as considerable mRNA expression of the 17beta-HSD types 3 and 4 in the human brain. In the present study, we report on 17beta-HSD type 5 mRNA expression in brain tissue of women and men. Data analysis did not reveal sex specific differences, but we determined a significantly higher mRNA concentration in the subcortical white matter (SC) than in the cerebral cortex (CX). Investigation of reductive 17beta-HSD in vitro activity with 2 microM androstenedione as the substrate revealed no sex specific differences. Testosterone formation was significantly higher in SC than in CX. Moreover, enzyme activity was significantly higher in brain tissue of adults compared to that of children.
- Subjects :
- 17-Hydroxysteroid Dehydrogenases metabolism
Adolescent
Adult
Aged
Androstenedione metabolism
Cerebral Cortex enzymology
Child
Child, Preschool
Female
Humans
Infant
Isoenzymes metabolism
Male
Middle Aged
NADP metabolism
Sex Characteristics
Temporal Lobe enzymology
Testosterone metabolism
Tissue Distribution
17-Hydroxysteroid Dehydrogenases genetics
Brain enzymology
Gene Expression
Isoenzymes genetics
RNA, Messenger analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0303-7207
- Volume :
- 171
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 11165025
- Full Text :
- https://doi.org/10.1016/s0303-7207(00)00432-9