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Increase of calmodulin III gene expression by mu-opioid receptor stimulation in PC12 cells.
- Source :
-
Japanese journal of pharmacology [Jpn J Pharmacol] 2000 Dec; Vol. 84 (4), pp. 412-7. - Publication Year :
- 2000
-
Abstract
- Calmodulin (CaM) is a principal multifunctional mediator of Ca2+ signaling in cells. It is reported that morphine increases CaM contents in mouse brain. However, the precise mechanism of CaM induction by morphine is unknown. We investigated the changes of CaM by opioid receptor stimulation in mRNA and protein levels. Expression of CaM was increased in dose- and time-dependent manners by morphine with RT-PCR assay in PC12 cells, and naloxone inhibited the effect of morphine. The expression was also increased with DAMGO (mu-opioid agonist), but not by DPDPE (delta) and U50488 (kappa). Northern blot analysis revealed that the CaMIII gene was responsive to morphine or DAMGO. CaM protein increased by DAMGO were distributed in both soluble and membranous fractions in the cells. Taken together, the data suggest that morphine induces the expression of CaMIII gene through mu-opioid receptor stimulation.
- Subjects :
- Animals
Calmodulin metabolism
Enkephalin, Ala(2)-MePhe(4)-Gly(5)- pharmacology
Gene Expression physiology
PC12 Cells
RNA, Messenger metabolism
Rats
Receptors, Opioid, mu metabolism
Analgesics, Opioid pharmacology
Calmodulin drug effects
Gene Expression drug effects
Morphine pharmacology
RNA, Messenger drug effects
Receptors, Opioid, mu drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0021-5198
- Volume :
- 84
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Japanese journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 11202613
- Full Text :
- https://doi.org/10.1254/jjp.84.412