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L-selectin inhibition does not reduce injury in a rabbit model of transient focal cerebral ischemia.
- Source :
-
Neurological research [Neurol Res] 2001 Jan; Vol. 23 (1), pp. 72-8. - Publication Year :
- 2001
-
Abstract
- Neutrophils are known to mediate injury in acute ischemic stroke especially during reperfusion. Migration of neutrophils into regions of ischemic injury involves binding to the endothelial cells via interactions with various adhesion molecules. One adhesion molecule, L-selectin, is found on the surface of leukocytes, and is shed prior to leukocyte infiltration. We studied whether a humanized antibody to L-selectin (HuDREG200) might limit ischemic injury in an experimental stroke model. New Zealand White rabbits underwent transorbital occlusion of the left middle cerebral, anterior cerebral and internal carotid arteries using aneurysm clips for 2 h followed by 6 h of reperfusion. Treatment with a saturating dose (4 mg kg-1) of HuDREG200 (n = 8) or vehicle (n = 8) was administered 20 min after occlusion and given as a single i.v. bolus. Hemispheric ischemic neuronal damage (IND) as seen on hematoxylin and eosin stained sections was no different between groups (HuDREG200, 23.3% +/- 6%; vehicle, 19.6% +/- 6%; mean +/- SEM, n.s., t-test). Immunohistochemical staining with neutrophil elastase confirmed the presence of neutrophils within regions of IND in control brains, but treatment did not alter their numbers within ischemic tissue. We conclude that antagonism of neutrophil adhesion at the level of L-selectin does not alter ischemic injury in experimental stroke.
- Subjects :
- Animals
Brain drug effects
Brain immunology
Brain physiopathology
Brain Ischemia physiopathology
Cell Movement drug effects
Cell Movement immunology
Disease Models, Animal
Leukocyte Count
Male
Nerve Degeneration drug therapy
Nerve Degeneration immunology
Nerve Degeneration physiopathology
Neutrophils cytology
Neutrophils immunology
Rabbits
Brain Ischemia drug therapy
Brain Ischemia immunology
L-Selectin immunology
L-Selectin metabolism
Neutrophils drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0161-6412
- Volume :
- 23
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neurological research
- Publication Type :
- Academic Journal
- Accession number :
- 11210435
- Full Text :
- https://doi.org/10.1179/016164101101198154