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Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network.

Authors :
Liljedahl M
Maeda Y
Colanzi A
Ayala I
Van Lint J
Malhotra V
Source :
Cell [Cell] 2001 Feb 09; Vol. 104 (3), pp. 409-20.
Publication Year :
2001

Abstract

When a kinase inactive form of Protein Kinase D (PKD-K618N) was expressed in HeLa cells, it localized to the trans-Golgi network (TGN) and caused extensive tubulation. Cargo that was destined for the plasma membrane was found in PKD-K618N-containing tubes but the tubes did not detach from the TGN. As a result, the transfer of cargo from TGN to the plasma membrane was inhibited. We have also demonstrated the formation and subsequent detachment of cargo-containing tubes from the TGN in cells stably expressing low levels of PKD-K618N. Our results suggest that PKD regulates the fission from the TGN of transport carriers that are en route to the cell surface.

Details

Language :
English
ISSN :
0092-8674
Volume :
104
Issue :
3
Database :
MEDLINE
Journal :
Cell
Publication Type :
Academic Journal
Accession number :
11239398
Full Text :
https://doi.org/10.1016/s0092-8674(01)00228-8