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Characterisation of nuclear ribosomal DNA sequences from Onchocerca volvulus and Mansonella ozzardi (Nematoda: Filarioidea) and development of a PCR-based method for their detection in skin biopsies.
- Source :
-
International journal for parasitology [Int J Parasitol] 2001 Feb; Vol. 31 (2), pp. 169-77. - Publication Year :
- 2001
-
Abstract
- The internal transcribed spacer region (ITS1, 5.8S gene and ITS2) of the two filarial nematodes Onchocerca volvulus and Mansonella ozzardi was sequenced, and two species-specific primers designed in the ITS2 to develop a PCR-based method for their specific detection and differentiation. When used with a universal reverse primer, the two species-specific primers gave amplification products of different size, which were readily separated in an agarose gel. The PCR was tested on skin biopsies from 51 people from three localities in Brazil where M. ozzardi is present, and results have been compared with those of parasitological examination of blood. The species-specific PCR gave a higher percentage of detection of infection by M. ozzardi than the parasitological examination of blood. No infection with O. volvulus was detected by PCR. This PCR-based assay may assist in determining the nature of infection in areas where both filarial species exist in sympatry.
- Subjects :
- Animals
Biopsy
DNA Primers
DNA, Helminth analysis
DNA, Helminth blood
Humans
Mansonella classification
Mansonella genetics
Mansonelliasis diagnosis
Mansonelliasis parasitology
Molecular Sequence Data
Onchocerca volvulus classification
Onchocerca volvulus genetics
Onchocerciasis diagnosis
Onchocerciasis parasitology
Sequence Analysis, DNA
Species Specificity
DNA, Ribosomal Spacer genetics
Mansonella isolation & purification
Onchocerca volvulus isolation & purification
Polymerase Chain Reaction methods
RNA, Ribosomal, 5.8S genetics
Skin parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7519
- Volume :
- 31
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal for parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 11239937
- Full Text :
- https://doi.org/10.1016/s0020-7519(00)00156-9