Back to Search Start Over

Bcl-2 gene and its family genes Bax, Bcl-Xl as well as Fas/Apo-1 and their clinical significance in acute leukemia.

Bcl-2 gene and its family genes Bax, Bcl-Xl as well as Fas/Apo-1 and their clinical significance in acute leukemia.

Authors :
Chen Y
Zhou J
Yue B
Xiang Z
Song X
Li C
Wang B
Source :
Chinese medical journal [Chin Med J (Engl)] 1998 Aug; Vol. 111 (8), pp. 682-5.
Publication Year :
1998

Abstract

Objective: To study the suppressing genes of apoptosis, namely Bcl-2, its family genes Bax, Bcl-Xl, and the inducing gene of apoptosis Fas/Apo-1.<br />Methods: The techniques of cytoimmuno-histiochemical stains, Western blotting and Northern blotting were used.<br />Results: It was found that the antigens of Bcl-2 in acute myelogenous leukemia (AML) and acute lymphocytic (ALL) was higher than that in the normal (P < 0.01). At same time, Bcl-2 was obviously lower expression in complete remission (CR) group than that in non-remission (NR) one by retrospective analysis (P < 0.01). Though Bcl-2 was low expression in CR with Western blotting, there was no statistical significance (P > 0.05). In CR group the expression of Bcl-2 mRNA was obviously lower than that of NR one (P < 0.01). Even though leukemia expression of Bax with cytoimmuno-histiochemical stain was also lower than that in the normal people, there was no difference between CR and NR with cytoimmuno-histiochemical, Western blotting and Northern blotting (P > 0.05). There was difference of Bcl-Xl mRNA in two groups (P < 0.01). The expression Fas/Apo-1 in leukemia was lower than that in normal people (P < 0.01). But in CR and NR, there was no difference with cytoimmuno-histiochemical stain and Western blotting.<br />Conclusions: The Changes of genes and their proteins are significant theoretically and clinically. The antigen expression of Bcl-2 and the expression of Bcl-2 mRNA may be considered as a prognostic index for AML.

Details

Language :
English
ISSN :
0366-6999
Volume :
111
Issue :
8
Database :
MEDLINE
Journal :
Chinese medical journal
Publication Type :
Academic Journal
Accession number :
11245018