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Bcl-2 gene and its family genes Bax, Bcl-Xl as well as Fas/Apo-1 and their clinical significance in acute leukemia.
Bcl-2 gene and its family genes Bax, Bcl-Xl as well as Fas/Apo-1 and their clinical significance in acute leukemia.
- Source :
-
Chinese medical journal [Chin Med J (Engl)] 1998 Aug; Vol. 111 (8), pp. 682-5. - Publication Year :
- 1998
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Abstract
- Objective: To study the suppressing genes of apoptosis, namely Bcl-2, its family genes Bax, Bcl-Xl, and the inducing gene of apoptosis Fas/Apo-1.<br />Methods: The techniques of cytoimmuno-histiochemical stains, Western blotting and Northern blotting were used.<br />Results: It was found that the antigens of Bcl-2 in acute myelogenous leukemia (AML) and acute lymphocytic (ALL) was higher than that in the normal (P < 0.01). At same time, Bcl-2 was obviously lower expression in complete remission (CR) group than that in non-remission (NR) one by retrospective analysis (P < 0.01). Though Bcl-2 was low expression in CR with Western blotting, there was no statistical significance (P > 0.05). In CR group the expression of Bcl-2 mRNA was obviously lower than that of NR one (P < 0.01). Even though leukemia expression of Bax with cytoimmuno-histiochemical stain was also lower than that in the normal people, there was no difference between CR and NR with cytoimmuno-histiochemical, Western blotting and Northern blotting (P > 0.05). There was difference of Bcl-Xl mRNA in two groups (P < 0.01). The expression Fas/Apo-1 in leukemia was lower than that in normal people (P < 0.01). But in CR and NR, there was no difference with cytoimmuno-histiochemical stain and Western blotting.<br />Conclusions: The Changes of genes and their proteins are significant theoretically and clinically. The antigen expression of Bcl-2 and the expression of Bcl-2 mRNA may be considered as a prognostic index for AML.
- Subjects :
- Adaptor Proteins, Signal Transducing
Apoptosis Regulatory Proteins
Carrier Proteins biosynthesis
Carrier Proteins genetics
Humans
Oncogene Protein v-cbl
Proto-Oncogene Proteins biosynthesis
Proto-Oncogene Proteins c-bcl-2 biosynthesis
Proto-Oncogene Proteins c-bcl-2 genetics
RNA, Messenger biosynthesis
RNA, Messenger genetics
Remission Induction
bcl-2-Associated X Protein
bcl-X Protein
Apoptosis
Genes, bcl-2
Leukemia, Myeloid, Acute genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics
Proto-Oncogene Proteins genetics
Retroviridae Proteins, Oncogenic biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0366-6999
- Volume :
- 111
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Chinese medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 11245018