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A novel osteoblast-derived C-type lectin that inhibits osteoclast formation.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2001 May 04; Vol. 276 (18), pp. 14916-23. Date of Electronic Publication: 2001 Feb 13. - Publication Year :
- 2001
-
Abstract
- We have cloned and expressed murine osteoclast inhibitory lectin (mOCIL), a 207-amino acid type II transmembrane C-type lectin. In osteoclast formation assays of primary murine calvarial osteoblasts with bone marrow cells, antisense oligonucleotides for mOCIL increased tartrate-resistant acid phosphatase-positive mononucleate cell formation by 3-5-fold, whereas control oligonucleotides had no effect. The extracellular domain of mOCIL, expressed as a recombinant protein in Escherichia coli, dose-dependently inhibited multinucleate osteoclast formation in murine osteoblast and spleen cell co-cultures as well as in spleen cell cultures treated with RANKL and macrophage colony-stimulating factor. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its inhibitory action on adherent spleen cell cultures, which were depleted of stromal and lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase of osteoclast formation and resulted in 70% inhibition during the differentiation phase. Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol, interleukin-1alpha and -11, and retinoic acid. In rodent tissues, Northern blotting, in situ hybridization, and immunohistochemistry demonstrated OCIL expression in osteoblasts and chondrocytes as well as in a variety of extraskeletal tissues. The overlapping tissue distribution of OCIL mRNA and protein with that of RANKL strongly suggests an interaction between these molecules in the skeleton and in extraskeletal tissues.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Bone Marrow Cells metabolism
DNA, Complementary
Humans
Immunohistochemistry
In Situ Hybridization
Lectins chemistry
Lectins genetics
Membrane Proteins chemistry
Membrane Proteins genetics
Mice
Molecular Sequence Data
RNA, Messenger genetics
Rats
Recombinant Proteins pharmacology
Sequence Homology, Amino Acid
Cell Division drug effects
Lectins pharmacology
Lectins, C-Type
Membrane Proteins pharmacology
Osteoblasts metabolism
Osteoclasts drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 276
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 11278931
- Full Text :
- https://doi.org/10.1074/jbc.M011554200