Back to Search
Start Over
Placental protein 14 induces apoptosis in T cells but not in monocytes.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2001 Jul 27; Vol. 276 (30), pp. 28268-73. Date of Electronic Publication: 2001 Apr 26. - Publication Year :
- 2001
-
Abstract
- Substantial evidence exists in literature to suggest that placental protein 14 (PP14) (recently renamed glycodelin A), exhibits immunosuppressive properties and is an indispensable macromolecule in the maternal system for the establishment, maintenance, and progression of pregnancy. Though there are several reports substantiating the above, the mechanism of its action at the molecular level has not been elucidated as yet. In this paper we provide data that suggest that amniotic fluid PP14 and recombinant PP14 expressed in Pichia pastoris induce apoptosis in human peripheral blood lymphocytes upon activation, independent of monocytes. That PP14 has a direct apoptotic action on T cells but not on monocytes was also demonstrated by utilizing human cell lines. PP14 was shown to induce apoptosis in the human T cell lines, Jurkat and MOLT-4 cells, but not in the human monocytic cell line, U937.
- Subjects :
- Acridine Orange pharmacology
Adult
Amniotic Fluid metabolism
Blotting, Western
Caspase 3
Caspases metabolism
Cell Division
Cell Line
Cell Separation
Chromatography, Gel
DNA Fragmentation
Ethidium pharmacology
Female
Flow Cytometry
Glycodelin
Humans
Immunosuppressive Agents pharmacology
Jurkat Cells
Lymphocytes metabolism
Male
Middle Aged
Pichia metabolism
Recombinant Proteins metabolism
T-Lymphocytes metabolism
U937 Cells
Apoptosis
Glycoproteins physiology
Monocytes pathology
Pregnancy Proteins physiology
T-Lymphocytes pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 276
- Issue :
- 30
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 11325960
- Full Text :
- https://doi.org/10.1074/jbc.M010487200