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Identification and analysis of the Georgia 98 serotype, a new serotype of infectious bronchitis virus.
- Source :
-
Avian diseases [Avian Dis] 2001 Jan-Mar; Vol. 45 (1), pp. 164-72. - Publication Year :
- 2001
-
Abstract
- Twenty-five field infectious bronchitis viruses (IBVs) similar to, but genetically distinct from, the DE072 serotype were isolated from several states in the United States from 1990 through 1999 and were examined molecularly and antigenically. A 421-bp sequence in the hypervariable region of the S1 gene was examined, and phylogenetic analysis on that region indicated that these viruses are closely related but fall into unique groups. Cross-virus neutralization testing and entire S1 sequence analysis on selected isolates further confirmed that fact, and we divided the viruses into the DE072 serotype and two other unique groups. In a vaccine protection trial, the commercially available DE072 vaccine showed less than 50% protection against viruses in one of the groups. The majority of the recent isolates belong to that group and share very low antigenic relatedness to the DE072 strain as well as other serotypes of IBV. Consequently, we designated this group as a new serotype, Georgia 98. We developed a restriction fragment length polymorphism analysis that can differentiate this new serotype from all other serotypes of IBV.
- Subjects :
- Animals
Antigenic Variation genetics
Chick Embryo
Chickens
DNA, Viral chemistry
Infectious bronchitis virus genetics
Neutralization Tests veterinary
Phylogeny
Polymorphism, Restriction Fragment Length
Reverse Transcriptase Polymerase Chain Reaction veterinary
Serotyping
United States
Viral Vaccines
Infectious bronchitis virus classification
Subjects
Details
- Language :
- English
- ISSN :
- 0005-2086
- Volume :
- 45
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Avian diseases
- Publication Type :
- Academic Journal
- Accession number :
- 11332478