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Novel alternatively spliced mRNA (1c) of the protein kinase A RIα subunit is implicated in haploid germ cell specific expression.
- Source :
-
Molecular reproduction and development [Mol Reprod Dev] 2001 May; Vol. 59 (1), pp. 11-6. - Publication Year :
- 2001
-
Abstract
- By using 5' RACE on rat testis cDNA we identified three alternatively spliced mRNAs of the RIalpha subunit of cAMP-dependent protein kinase that differed in their 5' untranslated regions. Two of these 5'-regions showed similarity with the human RIalpha exons 1a and 1b, while the third (1c) constituted a novel mRNA splice variant. Northern blot analysis showed that the 1c mRNA was specifically expressed in testis and only in postmeiotic germ cells. In contrast, the RIalpha 1b and RIalpha 1a mRNAs were present both in premeiotic germ cells and somatic cells of the testis, and the expression of both RIalpha 1a and 1b mRNAs were stimulated by cAMP in Sertoli cells. In sperm, the RIalpha protein was expressed after meiosis, and targeted to various subcellular structures via anchoring proteins. The RIalpha 1c haploid-specific mRNA, therefore, may be important for the regulation of RIalpha expression in sperm.
- Subjects :
- Animals
Base Sequence
Blotting, Northern
Cell Fractionation
Cloning, Molecular
Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
Cyclic AMP-Dependent Protein Kinases metabolism
Humans
Male
Molecular Sequence Data
Nucleic Acid Conformation
Protein Subunits
RNA, Messenger metabolism
Rats
Spermatozoa cytology
Testis cytology
Testis physiology
5' Untranslated Regions genetics
Alternative Splicing genetics
Cyclic AMP-Dependent Protein Kinases genetics
RNA, Messenger genetics
Spermatozoa metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1040-452X
- Volume :
- 59
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular reproduction and development
- Publication Type :
- Academic Journal
- Accession number :
- 11335941
- Full Text :
- https://doi.org/10.1002/mrd.1001