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Generation and mapping of expressed sequence tags from virus-infected swine macrophages.
- Source :
-
Animal biotechnology [Anim Biotechnol] 2001 May; Vol. 12 (1), pp. 51-67. - Publication Year :
- 2001
-
Abstract
- In an effort to identify genes that have a major effect on macrophage function during viral infection, we employed differential display reverse transcription (DDRT)-PCR to capture expressed sequence tags (ESTs) of swine alveolar macrophages infected by the porcine reproductive and respiratory syndrome virus (PRRSV). Sequence analyses showed that approximately 60% of these ESTs had significant similarity (> or =93%) to known pig ESTs or genes or matched sequences from other species with homology > or =80%. To determine chromosomal localization, PCR-based mapping was performed across either swine somatic cell hybrid or radiation hybrid panels. A total of 48 porcine viral response ESTs were mapped via the swine somatic cell panel or the INRA-Minnesota porcine Radiation Hybrid (IMpRH) panel (LOD > 6.0). Northern blot analyses confirmed PRRSV-induced altered transcript expression for several ESTs, including a 2'-5' oligoadenylate synthetase and a putative dual-specificity phosphatase. These virus-response ESTs represent good candidate genes for understanding PRRSV pathogenesis and for dissecting host genes which may have major effect on disease resistance.
- Subjects :
- 2',5'-Oligoadenylate Synthetase genetics
Animals
Base Sequence
Blotting, Northern
DNA Primers
Gene Expression Regulation
Molecular Sequence Data
Polymerase Chain Reaction
Porcine Reproductive and Respiratory Syndrome virology
Sequence Alignment
Sequence Analysis, DNA
Swine
Expressed Sequence Tags
Gene Expression Profiling
Macrophages, Alveolar virology
Porcine Reproductive and Respiratory Syndrome physiopathology
Porcine respiratory and reproductive syndrome virus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1049-5398
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Animal biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 11370681
- Full Text :
- https://doi.org/10.1081/ABIO-100102978