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Earlier expression of the transcription factor HFH-11B diminishes induction of p21(CIP1/WAF1) levels and accelerates mouse hepatocyte entry into S-phase following carbon tetrachloride liver injury.
- Source :
-
Hepatology (Baltimore, Md.) [Hepatology] 2001 Jun; Vol. 33 (6), pp. 1404-14. - Publication Year :
- 2001
-
Abstract
- Partial hepatectomy (PH) or toxic liver injury induces the proliferation of terminally differentiated hepatic cells to regenerate the original size of the adult liver. Previous PH liver regeneration studies showed that premature transgenic expression of the Forkhead Box M1b (FoxM1b, HFH-11B) transcription factor accelerated hepatocyte entry into DNA replication (S-phase). In this study, we used carbon tetrachloride (CCl(4)) liver injury to induce a different type of mouse liver regeneration and show that premature hepatic HFH-11B levels also accelerate the onset of hepatocyte S-phase in this injury model. Unlike PH liver regeneration, earlier hepatocyte proliferation after CCl(4) liver injury is correlated with diminished transgenic hepatic levels of p21(CIP1/WAF1) at the G1/S transition of the cell cycle. Differential hybridization of cDNA arrays and RNase protection studies determined that CCl(4) regenerating liver of transgenic mice displayed early stimulated expression of the S-phase promoting cyclin D1 and cyclin E and sustained levels of Cdc25a phosphatase genes. Compared with previous PH liver regeneration studies, our data suggest that premature expression of HFH-11B activates distinct S-phase promotion pathways in the CCl(4) liver injury model. Although proliferating transgenic hepatocytes induced by either PH or CCl(4) liver injury displayed early expression of identical M-phase cyclin genes (cyclin B1, B2, A2, and F), only CCl(4) regenerating transgenic liver exhibited earlier expression of the M-phase promoting Cdc25b. These studies suggest that CCl(4) injury of transgenic liver not only uses the same mechanisms as PH to mediate accelerated hepatocyte entry into mitosis, but also promotes M-phase entry by stimulating Cdc25b expression.
- Subjects :
- Animals
Carbon Tetrachloride
Cell Adhesion Molecules metabolism
Cell Cycle Proteins metabolism
Chemical and Drug Induced Liver Injury
Cyclin-Dependent Kinase Inhibitor p21
Cyclins metabolism
Forkhead Box Protein M1
Forkhead Transcription Factors
Liver Diseases physiopathology
Male
Mice
Mice, Inbred Strains
Mice, Transgenic genetics
Time Factors
Transcription Factors metabolism
cdc25 Phosphatases metabolism
Cyclins genetics
Gene Expression physiology
Gene Expression Regulation physiology
Hepatocytes physiology
Liver Diseases genetics
Liver Diseases pathology
S Phase
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0270-9139
- Volume :
- 33
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Hepatology (Baltimore, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 11391529
- Full Text :
- https://doi.org/10.1053/jhep.2001.24666