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Identification of androstenedione in a river containing paper mill effluent.
- Source :
-
Environmental toxicology and chemistry [Environ Toxicol Chem] 2001 Jun; Vol. 20 (6), pp. 1325-31. - Publication Year :
- 2001
-
Abstract
- Effluent from a paper mill discharging into the Fenholloway River, Taylor County, Florida, USA, contains chemicals that masculinize females of the resident population of eastern mosquitofish (Gambusia holbrooki), as evidenced in females by elongated anal fins, which is normally a male-specific trait. To identify androgenic components in the effluent, water collected from the Fenholloway River and a control tributary was fractionated using solid-phase extraction and reverse-phase high-performance-liquid chromatography. Two Fenholloway River fractions induced androgen receptor-dependent transcriptional activity in transient transfection cell culture assays. Of these, androstenedione was confirmed by liquid chromatography-mass spectrometry with multiple reaction monitoring.
- Subjects :
- Animals
Cells, Cultured
Chromatography, High Pressure Liquid
Female
Florida
Male
Mass Spectrometry
Receptors, Androgen genetics
Sex Characteristics
Spectrophotometry, Ultraviolet
Transcription, Genetic genetics
Androstenedione analysis
Cyprinodontiformes genetics
Industrial Waste analysis
Paper
Water Pollutants, Chemical analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0730-7268
- Volume :
- 20
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Environmental toxicology and chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 11392143
- Full Text :
- https://doi.org/10.1897/1551-5028(2001)020<1325:ioaiar>2.0.co;2