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Host metalloproteinases in Lyme arthritis.
- Source :
-
Arthritis and rheumatism [Arthritis Rheum] 2001 Jun; Vol. 44 (6), pp. 1401-10. - Publication Year :
- 2001
-
Abstract
- Objective: To assess the role of matrix metalloproteinases (MMPs) in cartilage and bone erosions in Lyme arthritis<br />Methods: We examined synovial fluid from 10 patients with Lyme arthritis for the presence of MMP-2, MMP-3, MMP-9, and "aggrecanase" activity using gelatinolytic zymography and immunoblot analysis. We developed an in vitro model of Lyme arthritis using cartilage explants and observed changes in cartilage degradation in the presence of Borrelia burgdorferi and/or various protease inhibitors.<br />Results: Synovial fluid from patients with Lyme arthritis was found to contain at least 3 MMPs: gelatinase A (MMP-2), stromelysin (MMP-3), and gelatinase B (MMP-9). In addition, there was evidence in 2 patients of "aggrecanase" activity not accounted for by the above enzymes. Infection of cartilage explants with B. burgdorferi resulted in induction of MMP-3, MMP-9, and "aggrecanase" activity. Increased induction of these enzymes by B. burgdorferi alone was not sufficient to cause cartilage destruction in the explants as measured by glycosaminoglycan (GAG) and hydroxyproline release. However, addition of plasminogen, which can act as an MMP activator, to cultures resulted in significant GAG and hydroxyproline release in the presence of B. burgdorferi. The MMP inhibitor batimastat significantly reduced the GAG release and completely inhibited the collagen degradation.<br />Conclusion: MMPs are found in synovial fluids from patients with Lyme arthritis and are induced from cartilage tissue by the presence of B. burgdorferi. Inhibition of MMP activity prevents B. burgdorferi-induced cartilage degradation in vitro.
- Subjects :
- Animals
Arthritis, Infectious etiology
Blotting, Western
Borrelia burgdorferi Group genetics
Borrelia burgdorferi Group isolation & purification
Cartilage chemistry
Cartilage cytology
Cattle
Culture Techniques
DNA, Bacterial analysis
Endopeptidases metabolism
Enzyme-Linked Immunosorbent Assay
Glycosaminoglycans analysis
Humans
Knee Joint enzymology
Knee Joint microbiology
Lyme Disease complications
Polymerase Chain Reaction
Synovial Fluid enzymology
Synovial Fluid microbiology
Arthritis, Infectious enzymology
Lyme Disease enzymology
Matrix Metalloproteinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0004-3591
- Volume :
- 44
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Arthritis and rheumatism
- Publication Type :
- Academic Journal
- Accession number :
- 11407701
- Full Text :
- https://doi.org/10.1002/1529-0131(200106)44:6<1401::AID-ART234>3.0.CO;2-S