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Tissue expression and subcellular localization of the pro-survival molecule Bcl-w.
- Source :
-
Cell death and differentiation [Cell Death Differ] 2001 May; Vol. 8 (5), pp. 486-94. - Publication Year :
- 2001
-
Abstract
- Anti-apoptotic members of the Bcl-2 family, such as Bcl-w, maintain cell viability by preventing the activation of the cell death effectors, the caspases. Gene targeting experiments in mice have demonstrated that Bcl-w is required for spermatogenesis and for survival of damaged epithelial cells in the gut. Bcl-w is, however, dispensable for physiological cell death in other tissues. Here we report on the analysis of Bcl-w protein expression using a panel of novel monoclonal antibodies. Bcl-w is found in a diverse range of tissues including colon, brain and testes. A survey of transformed cell lines and purified hematopoietic cells demonstrated that Bcl-w is expressed in cells of myeloid, lymphoid and epithelial origin. Subcellular fractionation and confocal laser scanning microscopy demonstrated that Bcl-w protein is associated with intracellular membranes. The implications of these results are discussed in the context of the phenotype of Bcl-w-null mice and recent data that suggest that Bcl-w may play a role in colon carcinogenesis.
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Antibody Specificity
Apoptosis Regulatory Proteins
Blotting, Western
Cell Line
Fluorescent Antibody Technique
Humans
Hybridomas cytology
Hybridomas immunology
Intracellular Membranes metabolism
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Precipitin Tests
Protein Transport
Proteins genetics
Proteins immunology
Proto-Oncogene Proteins c-bcl-2
Rats
Rats, Wistar
Transfection
Gene Expression Profiling
Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1350-9047
- Volume :
- 8
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cell death and differentiation
- Publication Type :
- Academic Journal
- Accession number :
- 11423909
- Full Text :
- https://doi.org/10.1038/sj.cdd.4400835