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Nup2p dynamically associates with the distal regions of the yeast nuclear pore complex.
- Source :
-
The Journal of cell biology [J Cell Biol] 2001 Jun 25; Vol. 153 (7), pp. 1465-78. - Publication Year :
- 2001
-
Abstract
- Nucleocytoplasmic transport is mediated by the interplay between soluble transport factors and nucleoporins resident within the nuclear pore complex (NPC). Understanding this process demands knowledge of components of both the soluble and stationary phases and the interface between them. Here, we provide evidence that Nup2p, previously considered to be a typical yeast nucleoporin that binds import- and export-bound karyopherins, dynamically associates with the NPC in a Ran-facilitated manner. When bound to the NPC, Nup2p associates with regions corresponding to the nuclear basket and cytoplasmic fibrils. On the nucleoplasmic face, where the Ran--GTP levels are predicted to be high, Nup2p binds to Nup60p. Deletion of NUP60 renders Nup2p nucleoplasmic and compromises Nup2p-mediated recycling of Kap60p/Srp1p. Depletion of Ran--GTP by metabolic poisoning, disruption of the Ran cycle, or in vitro by cell lysis, results in a shift of Nup2p from the nucleoplasm to the cytoplasmic face of the NPC. This mobility of Nup2p was also detected using heterokaryons where, unlike nucleoporins, Nup2p was observed to move from one nucleus to the other. Together, our data support a model in which Nup2p movement facilitates the transition between the import and export phases of nucleocytoplasmic transport.
- Subjects :
- Binding Sites physiology
Cell Nucleus metabolism
Cytoplasm metabolism
Deoxyglucose pharmacology
Fungal Proteins genetics
Fungal Proteins metabolism
Green Fluorescent Proteins
Guanosine Triphosphate metabolism
Heat-Shock Proteins metabolism
Luminescent Proteins genetics
Nuclear Proteins genetics
Porins genetics
Protein Binding drug effects
Protein Binding physiology
Protein Structure, Tertiary physiology
Protein Transport physiology
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Saccharomyces
Sodium Azide pharmacology
Staphylococcus aureus genetics
beta Karyopherins
ran GTP-Binding Protein metabolism
Nuclear Pore metabolism
Nuclear Pore Complex Proteins
Nuclear Proteins metabolism
Porins metabolism
Saccharomyces cerevisiae Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 153
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 11425876
- Full Text :
- https://doi.org/10.1083/jcb.153.7.1465