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Green fluorescent protein-Dal80p illuminates up to 16 distinct foci that colocalize with and exhibit the same behavior as chromosomal DNA proceeding through the cell cycle of Saccharomyces cerevisiae.
- Source :
-
Journal of bacteriology [J Bacteriol] 2001 Aug; Vol. 183 (15), pp. 4636-42. - Publication Year :
- 2001
-
Abstract
- Four GATA family DNA binding proteins mediate nitrogen catabolite repression-sensitive transcription in Saccharomyces cerevisiae. Gln3p and Gat1p are transcriptional activators, while Dal80p and Deh1p repress Gln3p- and Gat1p-mediated transcription by competing with these activators for binding to DNA. Strong Dal80p binding to DNA is thought to result from C-terminal leucine zipper-mediated dimerization. Many Dal80p binding site-homologous sequences are relatively evenly distributed across the S. cerevisiae genome, raising the possibility that Dal80p might be able to "stain" DNA. We demonstrate that cells containing enhanced green fluorescent protein-Dal80p (EGFP-Dal80p) exhibit up to 16 fluorescent foci that colocalize with DAPI (4',6'-diamidino-2-phenylindole)-positive material and follow DNA movement through the cell cycle, suggesting that EGFP-Dal80p may indeed be useful for monitoring yeast chromosomes in live cells and in real time.
- Subjects :
- Cell Cycle
Cell Cycle Proteins genetics
Cell Cycle Proteins metabolism
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Fluorescence
Fungal Proteins genetics
GATA Transcription Factors
Genes, Reporter
Green Fluorescent Proteins
Indoles metabolism
Luminescent Proteins genetics
Luminescent Proteins metabolism
Microscopy, Confocal methods
Microtubule Proteins genetics
Microtubule Proteins metabolism
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Repressor Proteins genetics
Saccharomyces cerevisiae genetics
Chromosomes, Fungal physiology
DNA, Fungal physiology
Fungal Proteins metabolism
Repressor Proteins metabolism
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins
Trans-Activators
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 183
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 11443099
- Full Text :
- https://doi.org/10.1128/JB.183.15.4636-4642.2001