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[An AFLP marker related to fibrogenesis in upland cotton (Gossypium hirsuturm L.)].
- Source :
-
Yi chuan xue bao = Acta genetica Sinica [Yi Chuan Xue Bao] 2001; Vol. 28 (7), pp. 677-82. - Publication Year :
- 2001
-
Abstract
- By using AFLP technique polymorphism analysis was performed between a fuzzlesslintless mutant line and its isogenic wild-type line, Xuzhou 142. Out of 6,360 bands produced by 64 pairs primers, a fragment, named as CF1, appearing stably in wild-type line, Xuzhou 142. This polymorphism was further verified using several normal fiber varieties and F2, F3 populations from the cross of fuzzless-lintless mutant line with a high-lint-percentage variety Yumian No. 1. The cosegregation of CF1 and fibrogenesis was proved, which suggested that CF1 can be used as a molecular marker for cotton fibrogenesis. The CF1 segment was cloned into PUCm-T Vector and then sequenced. The putative amino acid sequences, is an analogue to phenol hydroxylase alpha subunit, outer surface protein C, NADH dehydrogenase subunit 1, NADH-ubiquinone oxidoreductase, 2-oxoacid ferredoxin oxidoreduct and hypothetical 14.5kD protein.
Details
- Language :
- Chinese
- ISSN :
- 0379-4172
- Volume :
- 28
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Yi chuan xue bao = Acta genetica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 11480181