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Protective effect of green tea against benzo[a]pyrene-induced mutations in the liver of Big Blue transgenic mice.
- Source :
-
Mutation research [Mutat Res] 2001 Sep 01; Vol. 480-481, pp. 147-51. - Publication Year :
- 2001
-
Abstract
- We assessed the ability of green tea to protect against benzo[a]pyrene (B[a]P)-induced mutations in the liver of lacI transgenic male C57BL/6 Big Blue mice. The mice were given a 2% Japanese green tea hot water extract as their sole source of drinking water for 10 weeks. After 7 weeks, they received a total dose of 150 mg/kg B[a]P. Treatment with B[a]P resulted in a two-fold higher lacI mutant frequency than the untreated controls (8.6+/-0.8 x 10(-5) versus 4.0+/-0.7 x 10(-5), P=0.01). B[a]P increased the frequency of its characteristic mutation (GC-->TA transversions) nearly five-fold, from 0.75 x 10(-5) to 3.7 x 10(-5). In mice treated with green tea, the induced B[a]P mutant frequency decreased by 63%, while GC-->TA transversions were reduced by 54%. Thus, we report evidence that green tea extract significantly suppressed B[a]P-induced mutation by lowering its specific transversion mutation in the lacI transgene in vivo. Further studies will address the correlation between the modulation of metabolic enzymes and the protection against induced mutation by green tea.
- Subjects :
- Animals
Antimutagenic Agents chemistry
Benzo(a)pyrene toxicity
DNA Mutational Analysis
Genes, Reporter drug effects
Liver metabolism
Mice
Mice, Transgenic
Mutagenicity Tests
Plant Extracts chemistry
Antimutagenic Agents pharmacology
Liver drug effects
Mutation drug effects
Plant Extracts pharmacology
Tea chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0027-5107
- Volume :
- 480-481
- Database :
- MEDLINE
- Journal :
- Mutation research
- Publication Type :
- Academic Journal
- Accession number :
- 11506808
- Full Text :
- https://doi.org/10.1016/s0027-5107(01)00178-6