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Dynamics of antigen-specific helper T cells at the initiation of airway eosinophilic inflammation.
- Source :
-
European journal of immunology [Eur J Immunol] 2001 Sep; Vol. 31 (9), pp. 2669-79. - Publication Year :
- 2001
-
Abstract
- Bronchial asthma is characterized by chronic eosinophilic inflammation of the bronchial mucosa in which Th2 cells play crucial roles. Ovalbumin-reactive Th2 clones were labeled with a fluorescent-probe then infused into unprimed mice to elucidate the dynamics of antigen-specific T cells involved in allergic inflammation. Infiltration of not only labeled antigen-specific T cells, but also unlabeled nonspecific CD4(+) T cells into the bronchial mucosa following inhaled antigen challenge was detectable under confocal microscopy and flow cytometry. Accordingly, labeled T cells in the spleen were decreased, whereas those in hilar lymph nodes were increased upon antigen challenge. Approximately 45% of antigen-specific T cells that migrated into the lungs bore CD25, while another early activation marker, CD69, was expressed on 80% of the migrated T cells. Accordingly, antigen challenge to the mice induced in situ proliferation of antigen-specific T cells as well as bronchial epithelial cells in the lungs. Expression of vascular cell adhesion molecule (VCAM)-1, but not intercellular adhesion molecule (ICAM)-1, on the vascular endothelium in the lungs was enhanced following antigen challenge. Nevertheless, treatment with anti-VCAM-1 antibody, and also anti-ICAM-1 antibody strongly suppressed the accumulation of T cells, suggesting that both VCAM-1 and ICAM-1 are essential for antigen-stimulated T cell mobilization into peripheral tissues. Our current study visualized the kinetics and the mechanism of antigen-specific T cell migration in response to local challenge with a protein antigen.
- Subjects :
- Adoptive Transfer
Animals
Cell Movement
Clone Cells
Inflammation immunology
Intercellular Adhesion Molecule-1 physiology
Kinetics
Lung immunology
Lung ultrastructure
Lymphocyte Activation
Lymphoid Tissue immunology
Male
Mice
Mice, Inbred BALB C
Ovalbumin immunology
T-Lymphocytes, Helper-Inducer immunology
T-Lymphocytes, Helper-Inducer ultrastructure
Th2 Cells transplantation
Vascular Cell Adhesion Molecule-1 physiology
Asthma immunology
Pulmonary Eosinophilia immunology
Th2 Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 31
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 11536165
- Full Text :
- https://doi.org/10.1002/1521-4141(200109)31:9<2669::aid-immu2669>3.0.co;2-y