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The role of cellulases in the mechanism of changes of cell walls of Funaria hygrometrica moss protonema at clinostating.
- Source :
-
Advances in space research : the official journal of the Committee on Space Research (COSPAR) [Adv Space Res] 1992; Vol. 12 (1), pp. 99-102. - Publication Year :
- 1992
-
Abstract
- Using electroncytochemical and biochemical methods, differences between the cytochemical reaction intensity and activity of the cellulosolytic enzymes in Funaria hygrometrica moss cells grown for 30 days in the horizontal clinostat (2 rev/min) and in control have been studied. It has been shown that on clinostating the precipitate amount and size increases with the cellulase activity enhancement in the periplasmic space and protonema cell walls, when compared to control. Using biochemical methods it has been found that the activity of both endo-1,4-beta-glucanase and exo-1,4-beta-glucanase was higher under these conditions. A decrease of cellulose total content, its crystalline form, and pectic substances as well as an increase of hemicellulose content have been revealed in the clinostated material compared to control. Data obtained are discussed regarding the possible mechanism of cellulase activation and synthesis inhibition and cellulose crystallization in plant cell walls at clinostating.
- Subjects :
- Bryopsida chemistry
Bryopsida cytology
Bryopsida physiology
Cell Wall chemistry
Cell Wall enzymology
Cell Wall ultrastructure
Cellulase analysis
Crystallization
Glucan 1,4-beta-Glucosidase
Histocytochemistry
Microscopy, Electron
Polysaccharides analysis
Uridine Diphosphate Glucose metabolism
Weightlessness
beta-Glucosidase analysis
Bryopsida enzymology
Cell Wall physiology
Cellulase physiology
Gravitation
Rotation
beta-Glucosidase physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0273-1177
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Advances in space research : the official journal of the Committee on Space Research (COSPAR)
- Publication Type :
- Academic Journal
- Accession number :
- 11536995
- Full Text :
- https://doi.org/10.1016/0273-1177(92)90269-4