Back to Search
Start Over
D1' centers are less efficient than normal photosystem II centers.
- Source :
-
FEBS letters [FEBS Lett] 2001 Sep 07; Vol. 505 (1), pp. 113-7. - Publication Year :
- 2001
-
Abstract
- One prominent difference between the photosystem II (PSII) reaction center protein D1' in Synechocystis 6803 and normal D1 is the replacement of Phe-186 in D1 with leucine in D1'. Mutants of Synechocystis 6803 producing only D1', or containing engineered D1 proteins with Phe-186 substitutions, were analyzed by 77 K fluorescence emission spectra, chlorophyll a fluorescence induction yield and decay kinetics, and flash-induced oxygen evolution. Compared to D1-containing PSII centers, D1' centers exhibited a 50% reduction in variable chlorophyll a fluorescence yield, while the flash-induced O(2) evolution pattern was unaffected. In the F186 mutants, both the P680(+)/Q(A)(-) recombination and O(2) oscillation pattern were noticeably perturbed.
- Subjects :
- Amino Acid Substitution
Chlorophyll metabolism
Chlorophyll A
Fluorescence
Oxygen analysis
Oxygen metabolism
Phenylalanine
Photosynthetic Reaction Center Complex Proteins metabolism
Cyanobacteria physiology
Mutation
Photosynthetic Reaction Center Complex Proteins genetics
Photosystem II Protein Complex
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 505
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 11557052
- Full Text :
- https://doi.org/10.1016/s0014-5793(01)02794-6