Improved low-energy electron injection systems for high rate electron capture dissociation in Fourier transform ion cyclotron resonance mass spectrometry.

Authors :: Tsybin YO
Håkansson P
Budnik BA
Haselmann KF
Kjeldsen F
Gorshkov M
Zubarev RA
Source :: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2001; Vol. 15 (19), pp. 1849-54.
Publication Year :: 2001
Abstract: New low-energy electron injection systems based on indirectly heated dispenser cathodes facilitate electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance (FTICR) mass spectrometry. In this joint report, details are presented of the design and performance of these systems on two commercial FTICR instruments, 9.4 T Bruker BioAPEX in Uppsala and 4.7 T IonSpec Ultima in Odense. New results include obtaining meaningful one-scan MS/MS data for isolated precursor ions with millisecond irradiation times. The ECD rate improvement is not only due to the larger total electron current, but the larger emitting area as well.<br /> (Copyright 2001 John Wiley & Sons, Ltd.)

Subjects :: Amino Acid Sequence
Cyclotrons
Equipment Design
Fourier Analysis
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization instrumentation
Electrons
Mass Spectrometry instrumentation
Mass Spectrometry methods
Oligopeptides chemistry
Peptides chemistry
Spectrometry, Mass, Electrospray Ionization methods

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