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[Modified method of ultramicroelectrophoretic protein fractionation].

Authors :
Annenkov GA
Morozov VV
Dement'eva ES
Solntsev IuB
Source :
Tsitologiia [Tsitologiia] 1975 Sep; Vol. 17 (9), pp. 1098-103.
Publication Year :
1975

Abstract

A modification is described for the method of ultramicroelectrophoretic fractioning of proteins in polyacrylamide gel in capillaries 400--100 microns in diameter. The original construction of the assembly of apparatuses is offered which can be assembled from details made in the USSR. The operation of dosed filling of capillaries is significantly facilitated. The optimal quantity of protein in one sample reaches 1.5--0.1 mkg (depending on the capillary diameter). The reproducability and sharpness of protein fractions by the offered method are not worse compared with the generally accepted method of macro-disc electrophoresis.

Details

Language :
Russian
ISSN :
0041-3771
Volume :
17
Issue :
9
Database :
MEDLINE
Journal :
Tsitologiia
Publication Type :
Academic Journal
Accession number :
1166528