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Characterisation of a novel Mannheimia sp from Australian feedlot cattle.
- Source :
-
Australian veterinary journal [Aust Vet J] 2001 Sep; Vol. 79 (9), pp. 634-9. - Publication Year :
- 2001
-
Abstract
- Objective: To characterise eight isolates of a Gram-negative organism obtained from the upper respiratory tract of cattle showing evidence of mild upper respiratory tract disease.<br />Design: The isolates were compared with the five recognised species within the genus Mannheimia - M haemolytica, M glucosida, M granulomatis, M ruminalis and M varigena--using a range of phenotypic and genotypic methods.<br />Results: Phenotypic characterisation indicated that the isolates belonged to the trehalose-negative [Pasteurella] haemolytica complex. This complex has recently been reorganised into five species within the new genus Mannheimia. Ribotyping performed using HindIII and a computerised analysis system indicated that the eight Australian isolates formed a distinct cluster that was related to, but different from, the five recognised species of Mannheimia. The 16S rRNA sequence of one isolate (BNO311) was determined and a phylogenetic analysis performed. Isolate BNO311 was distinct from the five named Mannheimia spp but did join a larger cluster consisting of rRNA cluster IV (M varigena) and the unnamed rRNA cluster V of Mannheimia. DNA:DNA hybridisation between isolate BNO311 and M haemolytica NCTC 9380T, M granulomatis P411 and Actinobacillus ligniersii NCTC 4189T all suggested similarities of approximately 30%.<br />Conclusions: These phenotypic and genotypic characterisation studies suggest that the eight Australian isolates represent a new species of Mannheimia. Until further characterisation studies are performed, we are unwilling to propose a name for this taxon, preferring to refer to this possible new species as Bisgaard taxon 39 of cluster V of Mannheimia.
- Subjects :
- Animals
Australia
Base Composition
Base Sequence
Cattle
DNA, Bacterial chemistry
Genotype
Nasal Mucosa microbiology
Nucleic Acid Hybridization
Pasteurella genetics
Pasteurella isolation & purification
Pasteurella Infections microbiology
Phenotype
Phylogeny
RNA, Ribosomal, 16S genetics
Respiratory Tract Infections microbiology
Ribotyping veterinary
Cattle Diseases microbiology
Pasteurella classification
Pasteurella Infections veterinary
Respiratory Tract Infections veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 0005-0423
- Volume :
- 79
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Australian veterinary journal
- Publication Type :
- Academic Journal
- Accession number :
- 11702937
- Full Text :
- https://doi.org/10.1111/j.1751-0813.2001.tb10787.x