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Tumor necrosis factor modulates fibroblast apoptosis, PMN recruitment, and osteoclast formation in response to P. gingivalis infection.
Tumor necrosis factor modulates fibroblast apoptosis, PMN recruitment, and osteoclast formation in response to P. gingivalis infection.
- Source :
-
Journal of dental research [J Dent Res] 2001 Oct; Vol. 80 (10), pp. 1875-9. - Publication Year :
- 2001
-
Abstract
- P. gingivalis is an important oral pathogen, which has been closely linked to periodontal disease as well as lesions of endodontic origin. Both infections are associated with a decrease in fibroblast numbers, formation of an inflammatory infiltrate, and bone resorption. The goal of this study was to investigate the role that the host response plays in the capacity of P. gingivalis to stimulate fibroblast apoptosis, PMN recruitment, and osteoclastogenesis. This was accomplished by the use of an in vivo calvarial model in mice with targeted deletion of TNF receptors p55 and p75 and matched wild-type mice. The results indicate that P. gingivalis induces fibroblast apoptosis in vivo and establish for the first time that this involves the stimulation of a host response. Moreover, bacteria-stimulated PMN recruitment and osteoclastogenesis were also dependent upon the host response. The results suggest that much of the damage caused by P. gingivalis infection, including fibroblast apoptosis, at least under some circumstances, results from stimulation of the host response rather than the direct effect of bacterial products. Furthermore, this may represent a more general mechanism by which bacterial challenge induces apoptosis of matrix-producing cells through the induction of TNF.
- Subjects :
- Animals
Antigens, Bacterial genetics
Antigens, Bacterial immunology
Bacterial Proteins genetics
Bacterial Proteins physiology
Bacteroidaceae Infections immunology
Bone Resorption pathology
Bone Resorption physiopathology
Cell Count
Cell Differentiation physiology
Disease Models, Animal
Gene Deletion
Gene Expression Regulation
Membrane Proteins genetics
Membrane Proteins physiology
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Mice, Mutant Strains
Neutrophil Infiltration immunology
Periodontal Diseases microbiology
Periodontal Diseases physiopathology
Receptors, Tumor Necrosis Factor genetics
Receptors, Tumor Necrosis Factor physiology
Skull microbiology
Tumor Necrosis Factor-alpha immunology
Apoptosis physiology
Bacteroidaceae Infections physiopathology
Fibroblasts physiology
Neutrophil Infiltration physiology
Osteoclasts physiology
Porphyromonas gingivalis physiology
Tumor Necrosis Factor-alpha physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-0345
- Volume :
- 80
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of dental research
- Publication Type :
- Academic Journal
- Accession number :
- 11706944
- Full Text :
- https://doi.org/10.1177/00220345010800100301